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Human Prostaglandin D2 Synthase, Brain (PGD2S) ELISA Kit

SKU:
HUDL02272
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
A0A024R8G3
Sensitivity:
26.5pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
PTGDS,PDS,PGDS2,Prostaglandin-H2 D-Isomerase,Lipocalin-Type Prostaglandin D Synthase,Cerebrin-28,Beta-trace protein,Glutathione-independent PGD synthase
Reactivity:
Human
Sample Type:
Serum, Plasma, Cerebrospinal Fluid And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Prostaglandin D2 Synthase, Brain (PGD2S) ELISA Kit

The Human Prostaglandin D2 Synthase (Brain) ELISA Kit is specifically designed for the accurate detection of prostaglandin D2 synthase levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Prostaglandin D2 synthase is an important enzyme involved in the synthesis of prostaglandin D2, a lipid mediator that plays a key role in inflammation and allergic reactions.

Dysregulation of this enzyme has been linked to various inflammatory conditions, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies.With its reliable performance and ease of use, the Human Prostaglandin D2 Synthase (Brain) ELISA Kit is an essential tool for researchers looking to study the role of this enzyme in health and disease.

Product Name:Human Prostaglandin D2 Synthase, Brain (PGD2S) ELISA Kit
Product Code:HUDL02272
Size:96 Assays
Target:PGD2S
Synonyms:PTGDS, PDS, PGDS2, Prostaglandin-H2 D-Isomerase, Lipocalin-Type Prostaglandin D Synthase, Cerebrin-28, Beta-trace protein, Glutathione-independent PGD synthase
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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