The Human Prostacyclin Receptor (PTGIR) ELISA Kit is a powerful tool designed for the precise measurement of PTGIR levels in human samples including serum, plasma, and cell culture supernatants. This cutting-edge kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.The PTGIR receptor is a key component in the prostacyclin signaling pathway, playing a crucial role in regulating inflammation, vascular tone, and platelet aggregation. Dysregulation of PTGIR has been implicated in a range of diseases including cardiovascular disorders, pulmonary hypertension, and inflammatory conditions, highlighting its significance as a biomarker for disease severity and progression.
With the Human PTGIR ELISA Kit, researchers can delve deeper into the molecular mechanisms underlying PTGIR function, paving the way for novel therapeutic strategies and personalized medicine approaches. Elevate your research with this innovative kit and uncover new insights into the role of PTGIR in health and disease.
Product Name:
Human Prostacyclin Receptor (PTGIR) ELISA Kit
Product Code:
HUDL02445
Size:
96 Assays
Target:
PTGIR
Synonyms:
PTGI-R, IP1, PRIPR, Prostaglandin I2 Receptor, Prostanoid IP receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
