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Human Prosaposin (PSAP) ELISA Kit

SKU:
HUDL02425
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07602
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SAP1,GLBA,CSAct,Dispersin,Pro-Saposin,Proactivator polypeptide,Glucosylceramidase activator,Sulfatide/GM1 activator,Cerebroside sulfate activator,Sphingolipid Activator 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cerebrospinal Fluid, Seminal Plasma, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Prosaposin (PSAP) ELISA Kit

The Human Prosaposin (PSAP) ELISA Kit is a reliable and accurate tool for the detection of prosaposin levels in human serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Prosaposin, also known as PSAP, is a key protein involved in lipid metabolism and lysosomal storage disorders. It plays a critical role in cell growth, survival, and differentiation, making it a valuable biomarker for studying metabolic and lysosomal disorders, as well as potential therapeutic targets.

With the Human Prosaposin (PSAP) ELISA Kit, researchers can accurately measure prosaposin levels in biological samples, allowing for a better understanding of its role in disease pathogenesis and providing valuable insights for potential treatment strategies. Trust in this ELISA kit for precise and reliable results in your research endeavors.

Product Name:Human Prosaposin (PSAP) ELISA Kit
Product Code:HUDL02425
Size:96 Assays
Target:PSAP
Synonyms:SAP1, GLBA, CSAct, Dispersin, Pro-Saposin, Proactivator polypeptide, Glucosylceramidase activator, Sulfatide/GM1 activator, Cerebroside sulfate activator, Sphingolipid Activator 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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