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Human Prolylcarboxypeptidase (PRCP) ELISA Kit

SKU:
HUDL02390
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P42785
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PCP,HUMPCP,Lysosomal Pro-X Carboxypeptidase,Angiotensinase C,Lysosomal carboxypeptidase C,Proline carboxypeptidase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Prolylcarboxypeptidase (PRCP) ELISA Kit

The Human Prolylcarboxypeptidase (PRCP) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PRCP levels in human serum, plasma, and cell culture supernatants. This kit is ideal for a variety of research applications, providing reliable and reproducible results.PRCP is an important enzyme involved in various physiological processes, including blood pressure regulation, inflammation, and metabolism.

Dysregulation of PRCP has been implicated in diseases such as hypertension, diabetes, and obesity, making it a valuable biomarker for studying these conditions and developing new therapeutic strategies.With its advanced technology and ease of use, the Human Prolylcarboxypeptidase (PRCP) ELISA Kit is a valuable tool for researchers seeking to further understand the role of PRCP in health and disease.

Product Name:Human Prolylcarboxypeptidase (PRCP) ELISA Kit
Product Code:HUDL02390
Size:96 Assays
Target:PRCP
Synonyms:PCP, HUMPCP, Lysosomal Pro-X Carboxypeptidase, Angiotensinase C, Lysosomal carboxypeptidase C, Proline carboxypeptidase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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