Human Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) ELISA Kit
The Human Prolyl 4-Hydroxylase Alpha Polypeptide III (P4HA3) ELISA Kit is a powerful tool for the precise measurement of P4HA3 levels in human biological samples. This kit offers unparalleled sensitivity and specificity, ensuring accurate and dependable results for various research applications.P4HA3 is an important enzyme involved in collagen biosynthesis, playing a crucial role in maintaining the structural integrity of tissues and organs. Dysregulation of P4HA3 has been linked to various diseases, including fibrosis, cancer, and cardiovascular disorders, making it a valuable target for biomarker analysis and drug development.
With its high-performance capabilities, the Human P4HA3 ELISA Kit is an essential tool for researchers studying the molecular mechanisms underlying disease pathogenesis and exploring potential therapeutic interventions. Trust in the reliability and precision of this kit to advance your research efforts and uncover novel insights into P4HA3 biology.
Product Name:
Human Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) ELISA Kit
Product Code:
HUDL02164
Size:
96 Assays
Target:
P4Ha3
Synonyms:
Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide III
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.