Human Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2) ELISA Kit
The Human Prolyl 4-Hydroxylase Alpha Polypeptide II (P4HA2) ELISA Kit is a cutting-edge tool for the precise measurement of P4HA2 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, delivering accurate and consistent results for a variety of research applications.P4HA2 is a key enzyme involved in collagen synthesis and is essential for maintaining the structural integrity of tissues and organs. Dysregulation of P4HA2 has been linked to various diseases, including fibrosis, cancer, and wound healing disorders, highlighting its importance as a biomarker for disease progression and therapeutic development.
With the Human P4HA2 ELISA Kit, researchers can uncover valuable insights into the role of P4HA2 in disease pathogenesis and explore novel therapeutic strategies targeting this enzyme. Trust in this ELISA kit to provide reliable data for your scientific investigations and drive groundbreaking discoveries in the field of biomedical research.
Product Name:
Human Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2) ELISA Kit
Product Code:
HUDL02163
Size:
96 Assays
Target:
P4Ha2
Synonyms:
Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide II
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
