Human Proline/Serine Rich Coiled Coil protein 1 (PSRC1) ELISA Kit
The Human Proline Serine Rich Coiled-Coil Protein 1 (PSRC1) ELISA Kit is a highly reliable and accurate assay designed for the detection of PSRC1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and precise results for various research applications.PSRC1 is a key protein involved in cellular processes such as cell growth, differentiation, and apoptosis. It plays a critical role in numerous physiological and pathological conditions, including cancer, cardiovascular diseases, and neurological disorders, making it a valuable biomarker for disease research and therapeutic development.
With its advanced technology and ease of use, the Human PSRC1 ELISA Kit is an essential tool for researchers studying the molecular mechanisms underlying disease progression and exploring potential treatment strategies. Trust in this kit to provide accurate and reproducible data to advance your research goals.
Product Name:
Human Proline/Serine Rich Coiled Coil protein 1 (PSRC1) ELISA Kit
Product Code:
HUDL02439
Size:
96 Assays
Target:
PSRC1
Synonyms:
DDA3, Differential Display And Activated By p53
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
