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Human Prokineticin Receptor 1 (PKR1) ELISA Kit (HUDL02312)

SKU:
HUDL02312
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8TCW9
Sensitivity:
0.228ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
PROKR1,PKR-1,GPR73,GPR-73a,ZAQ,PK-R1,G Protein-Coupled Receptor 73,G-protein coupled receptor ZAQ
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Prokineticin Receptor 1 (PKR1) ELISA Kit

The Human Prokineticin Receptor 1 (PKR1) ELISA Kit is specifically designed for the accurate detection of PKR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PKR1 is a key receptor involved in various biological processes, including circadian rhythms, pain modulation, and immune responses.

Dysregulation of PKR1 has been linked to several diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With the Human Prokineticin Receptor 1 ELISA Kit, researchers can confidently measure PKR1 levels in their samples, opening up new possibilities for understanding the role of this receptor in health and disease.

Product Name:Human Prokineticin Receptor 1 (PKR1) ELISA Kit
Product Code:HUDL02312
Size:96 Assays
Target:PKR1
Synonyms:PROKR1, PKR-1, GPR73, GPR-73a, ZAQ, PK-R1, G Protein-Coupled Receptor 73, G-protein coupled receptor ZAQ
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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