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Human Programmed Cell Death Protein 5 (PDCD5) ELISA Kit

SKU:
HUDL02222
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O14737
Sensitivity:
0.18ng/mL
Range:
0.468-30ng/mL
ELISA Type:
Sandwich
Synonyms:
TFAR19,TFAR19 Novel Apoptosis-Related,TF1 Cell Apoptosis-Related Gene 19
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Human Programmed Cell Death Protein 5 (PDCD5) ELISA Kit

The Human Programmed Cell Death Protein 5 (PDCD5) ELISA Kit is a reliable and accurate tool for quantifying PDCD5 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for various research applications.PDCD5 is a critical protein involved in programmed cell death, playing a key role in regulating cell survival and apoptosis.

Dysregulation of PDCD5 has been linked to various diseases, including cancer and autoimmune disorders, making it a valuable biomarker for understanding these conditions and developing potential treatments.Overall, the Human PDCD5 ELISA Kit is an essential tool for researchers studying programmed cell death and its implications in disease pathology.

Product Name:Human Programmed Cell Death Protein 5 (PDCD5) ELISA Kit
Product Code:HUDL02222
Size:96 Assays
Target:PDCD5
Synonyms:TFAR19, TFAR19 Novel Apoptosis-Related, TF1 Cell Apoptosis-Related Gene 19
Detection Method:Sandwich
Range:0.468-30ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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