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Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

SKU:
HUDL02282
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8WXW3
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
PIBF,C13orf24,CEP90,Progesterone-Induced Blocking Factor 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

The Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit is a high-quality assay designed for the precise measurement of PIBF1 levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.PIBF1 is a critical protein that plays a key role in the modulation of immune responses and the regulation of progesterone levels in the body. Its dysregulation has been linked to various reproductive disorders and autoimmune conditions, making it a valuable biomarker for studying these diseases and developing targeted therapies.

With its advanced technology and user-friendly protocol, the Human PIBF1 ELISA Kit is a valuable tool for researchers and clinicians looking to investigate the role of PIBF1 in human health and disease. Trust Assay Genie for reliable and high-performance ELISA kits for your research needs.

Product Name:Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit
Product Code:HUDL02282
Size:96 Assays
Target:PIBF1
Synonyms:PIBF, C13orf24, CEP90, Progesterone-Induced Blocking Factor 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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