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Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit

SKU:
HUDL02170
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09466
Sensitivity:
1.8ng/mL
Range:
4.687-300ng/mL
ELISA Type:
Sandwich
Synonyms:
GD,GdA,GdF,GdS,PAEG,PEP,PP14,Glycodelin,Placental Protein 14,Alpha Uterine Protein,Pregnancy-Associated Endometrial Alpha-2-Globulin,Progesterone-associated
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit

The Human Progestagen-Associated Endometrial Protein (PAEP) ELISA Kit is specifically designed to accurately measure PAEP levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research purposes.PAEP, also known as glycodelin, plays a key role in the regulation of female reproductive processes, including implantation and maintaining pregnancy.

Abnormal PAEP levels have been associated with conditions such as infertility, miscarriage, and certain gynecological disorders.With its reliable performance and broad range of applications, the Human PAEP ELISA Kit is an essential tool for studying the role of PAEP in reproductive health and developing potential diagnostic and therapeutic strategies.

Product Name:Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit
Product Code:HUDL02170
Size:96 Assays
Target:PAEP
Synonyms:GD, GdA, GdF, GdS, PAEG, PEP, PP14, Glycodelin, Placental Protein 14, Alpha Uterine Protein, Pregnancy-Associated Endometrial Alpha-2-Globulin, Progesterone-associated
Detection Method:Sandwich
Range:4.687-300ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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