Human Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) ELISA Kit
The Human Procollagen Lysine 1,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) ELISA Kit is a cutting-edge tool designed for the precise measurement of PLOD1 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it invaluable for a variety of research purposes.PLOD1, also known as lysyl hydroxylase 1, is a key enzyme involved in collagen biosynthesis, specifically in the hydroxylation of lysine residues within collagen molecules. Dysregulation of PLOD1 has been linked to various connective tissue disorders, such as Ehlers-Danlos Syndrome, and fibrotic diseases, highlighting its significance as a biomarker in understanding and treating these conditions.
By utilizing the Human PLOD1 ELISA Kit, researchers can gain critical insights into the role of PLOD1 in collagen synthesis and its implications for various diseases. This kit enables precise quantification of PLOD1 levels, providing valuable data for further investigation and potential therapeutic targeting. Elevate your research with the advanced capabilities of the Human PLOD1 ELISA Kit.
Product Name:
Human Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
