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Human Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit

SKU:
HUDL02290
Product Type:
ELISA Kit
Sensitivity:
0.62ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
P2NP,N-Propeptide Of Type II Procollagen,Procollagen II Amino Terminal Propeptide
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit

The Human Procollagen II N-terminal propeptide (PIIINP) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of PIIINP levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.PIIINP is a key biomarker for collagen synthesis, particularly type II collagen, which is essential for the structural integrity of cartilage and other connective tissues.

Its levels are indicative of processes related to tissue repair, fibrosis, and inflammation, making it valuable for studying conditions such as arthritis, osteoarthritis, and fibrotic diseases.Overall, the Human Procollagen II N-terminal propeptide (PIIINP) ELISA Kit offers a valuable tool for researchers and clinicians interested in studying collagen metabolism, tissue repair, and various pathological conditions involving connective tissues.

Product Name:Human Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit
Product Code:HUDL02290
Size:96 Assays
Target:PIINP
Synonyms:P2NP, N-Propeptide Of Type II Procollagen, Procollagen II Amino Terminal Propeptide
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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