The Human Procollagen I (PCI) ELISA Kit is specifically designed for the precise measurement of procollagen I levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it an excellent tool for various research purposes.Procollagen I is a key protein involved in collagen synthesis, playing a vital role in maintaining structural integrity and elasticity of connective tissues. Dysregulation of procollagen I production is linked to several health conditions, including fibrosis, osteogenesis imperfecta, and skin disorders.
As such, procollagen I serves as a critical biomarker in understanding these pathological processes and exploring potential therapeutic interventions.By utilizing the Human Procollagen I (PCI) ELISA Kit, researchers can gain valuable insights into the regulation of collagen metabolism and its implications in various diseases. This kit offers a convenient and reliable method for measuring procollagen I levels, enabling accurate assessment of disease progression and treatment efficacy.
Product Name:
Human Procollagen I (PCI) ELISA Kit
Product Code:
HUDL02204
Size:
96 Assays
Target:
PCI
Synonyms:
PCOL-I, PC-I, PC1, Type I Procollagen
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
