Human Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit
SKU:
HUDL02210
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15113
Sensitivity:
0.238ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
PCOLCE,PCPE,Procollagen Type 1 COOH-Terminal Proteinase Enhancer,Procollagen C Endopeptidase Enhancer,Type I procollagen COOH-terminal proteinase enhancer
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Human Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit
The Human Procollagen C-Proteinase Enhancer 1 (PCPE1) ELISA Kit is a valuable tool for researchers seeking to accurately measure levels of PCPE1 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PCPE1 is a critical protein involved in the regulation of collagen synthesis, specifically in the processing of procollagen molecules. Dysregulation of PCPE1 has been linked to various health conditions, including fibrosis, cardiovascular diseases, and connective tissue disorders.
Therefore, the accurate measurement of PCPE1 levels is essential for understanding disease mechanisms and potential therapeutic interventions.With its reliable performance and easy-to-use format, the Human PCPE1 ELISA Kit is an essential tool for researchers investigating the role of PCPE1 in health and disease. Order yours today and take your research to the next level.
Product Name:
Human Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit
Product Code:
HUDL02210
Size:
96 Assays
Target:
PCPE1
Synonyms:
PCOLCE, PCPE, Procollagen Type 1 COOH-Terminal Proteinase Enhancer, Procollagen C Endopeptidase Enhancer, Type I procollagen COOH-terminal proteinase enhancer
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
