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Human Pregnane X Receptor (PXR) ELISA Kit

SKU:
HUDL02472
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O75469
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NR1I2,SAR,PAR1,PAR2,PRR,BXR,ONR1,PAR,PARq,SXR,Nuclear Receptor Subfamily 1,Group I,Member 2,Steroid and xenobiotic receptor,Pregnane X receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Pregnane X Receptor (PXR) ELISA Kit

The Human Pregnane X Receptor (PXR) ELISA Kit is a powerful tool for researchers looking to accurately detect levels of PXR in human biological samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of applications.PXR is a key protein involved in the regulation of drug metabolism and detoxification in the liver, as well as playing a role in the regulation of inflammation and immunity.

Dysregulation of PXR activity has been linked to various diseases, including cancer, inflammatory disorders, and metabolic syndromes, highlighting its importance as a potential therapeutic target.By utilizing the Human PXR ELISA Kit, researchers can gain valuable insights into the role of PXR in disease pathogenesis and drug response, ultimately leading to advancements in patient care and drug development.

Product Name:Human Pregnane X Receptor (PXR) ELISA Kit
Product Code:HUDL02472
Size:96 Assays
Target:PXR
Synonyms:NR1I2, SAR, PAR1, PAR2, PRR, BXR, ONR1, PAR, PARq, SXR, Nuclear Receptor Subfamily 1,Group I,Member 2, Steroid and xenobiotic receptor, Pregnane X receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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