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Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit

SKU:
HUDL02429
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11465
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CEA,PSG1,PSBG2,PSGGB,Carcinoembryonic Antigen SG8,Pregnancy-specific glycoprotein 2,Pregnancy-specific beta-1 glycoprotein E
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit

The Human Pregnancy-Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit is a reliable tool for the precise measurement of PSG2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PSG2 is a protein that is specifically expressed during pregnancy and plays a crucial role in maternal-fetal interactions. Studying PSG2 levels can provide valuable insights into pregnancy-related conditions and complications, making it an important biomarker for research in obstetrics and gynecology.

With its advanced technology and user-friendly design, the Human PSG2 ELISA Kit from Assay Genie is the perfect choice for researchers looking to explore the role of PSG2 in pregnancy and women's health. Unlock the potential of PSG2 research with this high-quality ELISA kit.

Product Name:Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit
Product Code:HUDL02429
Size:96 Assays
Target:PSG2
Synonyms:CEA, PSG1, PSBG2, PSGGB, Carcinoembryonic Antigen SG8, Pregnancy-specific glycoprotein 2, Pregnancy-specific beta-1 glycoprotein E
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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