Human PRDX4 Recombinant Protein (RPPB2180)
- SKU:
- RPPB2180
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13162
- Research Area:
- Enzymes
Description
| Product Name: | Human PRDX4 Recombinant Protein |
| Product Code: | RPPB2180 |
| Size: | 25µg |
| Species: | Human |
| Target: | PRDX4 |
| Synonyms: | EC 1.11.1.15, AOE37-2, Peroxiredoxin-IV, Prx-IV, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372, Antioxidant enzyme AOE372. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | PRDX4 Human solution containing 20mM Tris-HCl pH-8 & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN |
| Biological Activity: | Specific activity: approximately 230-310 pmole/min/�g.Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute. |
PRDX4 is an antioxidant enzyme that is part of the peroxiredoxin family. PRDX4 is localized to the cytoplasm. PRDX4 reduces hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. PRDX4 has a regulatory part in the activation of the transcription factor NF-kappaB. PRDX4 participates in redox regulation of the cell. PRDX4 regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
PRDX4 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 255 amino acids (38-271 a.a.) and having a molecular mass of 28.8kDa. PRDX4 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography.
| UniProt Protein Function: | PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.11.1.15; Secreted; Mitochondrial; Secreted, signal peptide Chromosomal Location of Human Ortholog: Xp22.11 Cellular Component: cytosol; extracellular space; mitochondrion; nucleus; smooth endoplasmic reticulum Molecular Function:protein binding; protein homodimerization activity; thioredoxin peroxidase activity Biological Process: 4-hydroxyproline metabolic process; cell redox homeostasis; extracellular matrix organization and biogenesis; I-kappaB phosphorylation; male gonad development; protein maturation via protein folding; spermatogenesis |
| NCBI Summary: | The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13162 |
| NCBI GenInfo Identifier: | 3024727 |
| NCBI Gene ID: | 10549 |
| NCBI Accession: | Q13162.1 |
| UniProt Secondary Accession: | Q13162,Q6FHT3, |
| UniProt Related Accession: | Q13162 |
| Molecular Weight: | |
| NCBI Full Name: | Peroxiredoxin-4 |
| NCBI Synonym Full Names: | peroxiredoxin 4 |
| NCBI Official Symbol: | PRDX4�� |
| NCBI Official Synonym Symbols: | PRX-4; AOE372; AOE37-2; HEL-S-97n�� |
| NCBI Protein Information: | peroxiredoxin-4 |
| UniProt Protein Name: | Peroxiredoxin-4 |
| UniProt Synonym Protein Names: | Antioxidant enzyme AOE372; AOE37-2; Peroxiredoxin IV; Prx-IV; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372 |
| Protein Family: | Peroxiredoxin |
| UniProt Gene Name: | PRDX4�� |
| UniProt Entry Name: | PRDX4_HUMAN |
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