Human PPP1R1A (Protein Phosphatase 1 Regulatory Subunit A) ELISA Kit
The Human PPP1R1A (Protein Phosphatase 1 Regulatory Subunit A) ELISA Kit is a specialized assay designed for the quantitative analysis of PPP1R1A levels in human biological samples. PPP1R1A, also known as DARPP-32, is a critical regulatory subunit of protein phosphatase 1 that plays a crucial role in signal transduction pathways, neural function, and synaptic plasticity. It acts as a key modulator of protein phosphorylation states in response to neurotransmitters and hormones, impacting various cellular processes and behaviors. This ELISA kit provides researchers with a reliable method to quantify PPP1R1A expression, offering insights into its involvement in neurobiological functions, neuronal signaling pathways, and neuropsychiatric disorders.
By accurately measuring PPP1R1A levels, researchers can gain a better understanding of the intricate regulatory mechanisms controlled by this protein and its implications in cognitive processes and brain-related conditions. The Human PPP1R1A ELISA Kit ensures exceptional sensitivity and specificity, delivering reproducible and dependable results. With its high-quality performance and user-friendly protocols, this kit is a valuable tool for researchers investigating the role of PPP1R1A in neurological function and associated diseases, offering insights into potential therapeutic targets and diagnostic biomarkers.
Product Name:
Human PPP1R1A (Protein Phosphatase 1 Regulatory Subunit A) ELISA Kit
SKU:
AEES00698
Target:
Human PPP1R1A (Protein Phosphatase 1 Regulatory Subunit A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPP1R1A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPP1R1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPP1R1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PPP1R1A. You can calculate the concentration of Human PPP1R1A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
86-100
90-101
Average (%)
95
91
95
1:4
Range (%)
85-101
86-101
85-99
Average (%)
92
93
90
1:8
Range (%)
92-105
79-93
90-102
Average (%)
97
86
95
1:16
Range (%)
87-99
81-95
85-98
Average (%)
92
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.1
558.59
1997.49
186.36
584.37
1844.11
Standard deviation
9.75
27.65
61.92
11.0
27.35
92.94
C V (%)
5.13
4.95
3.1
5.9
4.68
5.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PPP1R1A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PPP1R1A in samples. No significant cross-reactivity or interference between Human PPP1R1A and analogues was observed.
