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Human PPM1F Recombinant Protein (RPPB2158)

SKU:
RPPB2158
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49593
Research Area:
Enzymes
€309
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Description

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Product Name:Human PPM1F Recombinant Protein
Product Code:RPPB2158
Size:20µg
Species:Human
Target:PPM1F
Synonyms:Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PPM1F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS

Protein Phosphatase 1F (PPM1F) is a part of the PP2C family of Ser/Thr protein phosphatases whose members are known to be negative regulators of cell stress response pathways. PPM1F interacts with Rho guanine nucleotide exchange factors (PIX), and as a result blocks the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. �Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is one of the substrates of PPM1F. PPM1F is found to be an apoptosis Promoter.

PPM1F Human Recombinant produced in E. coli is a single polypeptide chain containing 477 amino acids (1-454) and having a molecular mass of 52kDa.PPM1F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PPM1F: a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members can be negative regulators of cell stress response pathways. Interact with PAK-interacting exchange factor beta (ARHGEF7), blocking the effects PAK1, a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CaMK2-gamma) is one of the substrates of this phosphatase. Promotes apoptosis.
UniProt Protein Details:

Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: cytosol; protein complex

Molecular Function:calmodulin-dependent protein phosphatase activity; phosphoric monoester hydrolase activity; protein binding; protein serine/threonine phosphatase activity

Biological Process: negative regulation of peptidyl-serine phosphorylation; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of chemotaxis; positive regulation of focal adhesion formation; positive regulation of growth; positive regulation of stress fiber formation

NCBI Summary:The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P49593
NCBI GenInfo Identifier:24638458
NCBI Gene ID:9647
NCBI Accession:P49593.3
UniProt Secondary Accession:P49593,Q96PM2, A8K6G3, B7Z2C3,
UniProt Related Accession:P49593
Molecular Weight:38,518 Da
NCBI Full Name:Protein phosphatase 1F
NCBI Synonym Full Names:protein phosphatase, Mg2+/Mn2+ dependent 1F
NCBI Official Symbol:PPM1F��
NCBI Official Synonym Symbols:CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase��
NCBI Protein Information:protein phosphatase 1F
UniProt Protein Name:Protein phosphatase 1F
UniProt Synonym Protein Names:Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog; hFem-2
Protein Family:Protein phosphatase
UniProt Gene Name:PPM1F��
UniProt Entry Name:PPM1F_HUMAN

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