Human PPIH Recombinant Protein (RPPB1546)
- SKU:
- RPPB1546
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O43447
- Research Area:
- Enzymes
Description
| Product Name: | Human PPIH Recombinant Protein |
| Product Code: | RPPB1546 |
| Size: | 20µg |
| Species: | Human |
| Target: | PPIH |
| Synonyms: | Oeptidylprolyl Isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, Peptidyl-prolyl cis-trans isomerase H, PPIase H, Rotamase H, U-snRNP-associated cyclophilin SnuCyp-20, USA-CYP, Small nuclear ribonucleoprotein particle-specific cyclophilin H, peptidylprolyl isomerase H, CYP-20, MGC5016, Cyclophilin-H. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 1 mg/ml solution containing 1x PBS pH-7.4 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGEFRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM |
| Biological Activity: | Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
PPIH is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and increase protein folding. PPIH enzyme is a precise factor of the complex that comprises pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. PPIH possess PPIase activity and acts as a protein chaperone that mediates the interactions between different proteins inside the spliceosome.
PPIH Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids (1-177) and having a molecular mass of 19.2 kDa. PPIH is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPIH: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone. Belongs to the cyclophilin-type PPIase family. PPIase H subfamily. |
| UniProt Protein Details: | Protein type:Cyclophilin; EC 5.2.1.8; Isomerase; RNA processing; Spliceosome Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: cytoplasm; nuclear speck; nucleoplasm; spliceosome; U4/U6 x U5 tri-snRNP complex Molecular Function:cyclosporin A binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; ribonucleoprotein binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of viral genome replication; protein complex assembly |
| NCBI Summary: | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43447 |
| NCBI GenInfo Identifier: | 37082225 |
| NCBI Gene ID: | 10465 |
| NCBI Accession: | O43447.1 |
| UniProt Secondary Accession: | O43447,A6NNE7, |
| UniProt Related Accession: | O43447 |
| Molecular Weight: | 19kDa |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase H |
| NCBI Synonym Full Names: | peptidylprolyl isomerase H |
| NCBI Official Symbol: | PPIH�� |
| NCBI Official Synonym Symbols: | CYPH; CYP-20; USA-CYP; SnuCyp-20�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase H |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase H |
| UniProt Synonym Protein Names: | Rotamase H; Small nuclear ribonucleoprotein particle-specific cyclophilin H; CypH; U-snRNP-associated cyclophilin SnuCyp-20; USA-CYP |
| UniProt Gene Name: | PPIH�� |
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