Human PPIF Recombinant Protein (RPPB1542)
- SKU:
- RPPB1542
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P30405
- Research Area:
- Enzymes
Description
| Product Name: | Human PPIF Recombinant Protein |
| Product Code: | RPPB1542 |
| Size: | 50µg |
| Species: | Human |
| Target: | PPIF |
| Synonyms: | Oeptidylprolyl Isomerase F, PPIF, CYP-D, CYP3, SnuCyp-20, Peptidyl-prolyl cis-trans isomerase mitochondrial, Cyclophilin F, FLJ90798, MGC117207, peptidylprolyl isomerase F. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | PPIF solution containing 20mM Tris-HCl pH-7.5, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS |
| Biological Activity: | Specific activity is > 250 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
PPIF is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIF accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
PPIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 198 amino acids (30-207) and having a molecular mass of 21 kDa. The PPIF is fused to a 20 amino acid His tag at N-terminus and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. |
| UniProt Protein Details: | Protein type:EC 5.2.1.8; Isomerase; Mitochondrial Chromosomal Location of Human Ortholog: 10q22.3 Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex Molecular Function:peptidyl-prolyl cis-trans isomerase activity; protein binding Biological Process: negative regulation of apoptosis; negative regulation of ATPase activity; regulation of mitochondrial membrane permeability |
| NCBI Summary: | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30405 |
| NCBI GenInfo Identifier: | 231968 |
| NCBI Gene ID: | 10105 |
| NCBI Accession: | P30405.1 |
| UniProt Secondary Accession: | P30405,Q2YDB7, Q5W131, |
| UniProt Related Accession: | P30405 |
| Molecular Weight: | 16,541 Da |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase F, mitochondrial |
| NCBI Synonym Full Names: | peptidylprolyl isomerase F |
| NCBI Official Symbol: | PPIF�� |
| NCBI Official Synonym Symbols: | CYP3; CypD; CyP-M; Cyp-D�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase F, mitochondrial |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase F, mitochondrial |
| UniProt Synonym Protein Names: | Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F |
| Protein Family: | Peptidyl-prolyl cis-trans isomerase |
| UniProt Gene Name: | PPIF�� |
| UniProt Entry Name: | PPIF_HUMAN |
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