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Human POTE Ankyrin Domain Family, Member G (POTEG) ELISA Kit (HUDL02369)

SKU:
HUDL02369
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6S5H5
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
A26C2,POTE14,POTE14a,CT104.4,ANKRD26-Like Family C Member 2,Cancer/Testis Antigen Family 104,Member 4,Prostate, ovary, testis-expressed protein on chromosome 14
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749

Description

system_update_altDatasheet

Human POTE Ankyrin Domain Family, Member G (POTEG) ELISA Kit

The Human ANG (Angiogenin) ELISA Kit is specifically designed to detect and quantify levels of angiogenin in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for a wide range of research applications.Angiogenin is a key protein involved in angiogenesis, the process of forming new blood vessels, and plays a crucial role in cell proliferation.

Dysregulation of angiogenin has been linked to various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a vital biomarker for studying these diseases and potential therapeutic interventions.With the Human ANG ELISA Kit, researchers can confidently measure angiogenin levels in human samples, advancing our understanding of the role of angiogenin in health and disease.

Product Name:Human POTE Ankyrin Domain Family, Member G (POTEG) ELISA Kit
Product Code:HUDL02369
Size:96 Assays
Target:POTEG
Synonyms:A26C2, POTE14, POTE14a, CT104.4, ANKRD26-Like Family C Member 2, Cancer/Testis Antigen Family 104,Member 4, Prostate, ovary, testis-expressed protein on chromosome 14
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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