Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N,Member 2 (KCNN2) ELISA Kit
The Human KCNN2 (Potassium Intermediate/ Small Conductance Calcium-Activated Channel, Subfamily N, Member 2) ELISA Kit is a powerful tool for the precise measurement of KCNN2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research purposes.KCNN2 is a vital protein involved in the regulation of potassium ion flow and cellular excitability. It plays a key role in various physiological processes and has been implicated in neurological disorders, cardiovascular diseases, and cancer.
By measuring KCNN2 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and explore potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human KCNN2 ELISA Kit. Its reliability and precision make it an indispensable tool for studying the complex functions of KCNN2 and its implications in disease pathology. Order yours today and unlock new possibilities in the field of biomedical research.
Product Name:
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N,Member 2 (KCNN2) ELISA Kit
Product Code:
HUDL01601
Size:
96 Assays
Target:
KCNN2
Synonyms:
KCa2.2, SK2, SKCA2, hSK2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
