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Human Potassium Chloride Cotransporters 4 (KCC4) ELISA Kit

SKU:
HUDL01598
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y666
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC12A7,Solute Carrier Family 12(Potassium/Chloride Transporters)Member 7,Electroneutral potassium-chloride cotransporter 4,K-Cl cotransporter 4
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Potassium Chloride Cotransporters 4 (KCC4) ELISA Kit

The Human Potassium Chloride Cotransporters 4 (KCC4) ELISA Kit is a powerful tool for the quantitative measurement of KCC4 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications.KCC4 is a key transporter protein involved in the regulation of potassium and chloride ions in cells, playing a crucial role in various physiological processes including cell volume regulation and neurotransmission.

Dysregulation of KCC4 has been associated with conditions such as epilepsy, hypertension, and neuronal excitability disorders, making it a valuable target for research and potential therapeutic interventions.The Human Potassium Chloride Cotransporters 4 (KCC4) ELISA Kit provides researchers with a reliable and efficient tool to study the role of KCC4 in health and disease, offering insight into potential biomarkers and therapeutic targets for a range of conditions.

Product Name:Human Potassium Chloride Cotransporters 4 (KCC4) ELISA Kit
Product Code:HUDL01598
Size:96 Assays
Target:KCC4
Synonyms:SLC12A7, Solute Carrier Family 12(Potassium/Chloride Transporters)Member 7, Electroneutral potassium-chloride cotransporter 4, K-Cl cotransporter 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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