Human Potassium Chloride Cotransporters 2 (KCC2) ELISA Kit
The Human Potassium Chloride Cotransporters 2 (KCC2) ELISA Kit is specifically designed for the accurate and reliable detection of KCC2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for precise measurements and reproducible results, making it an excellent tool for various research applications.KCC2 is a critical transporter protein involved in the regulation of neuronal chloride homeostasis and plays a key role in inhibitory neurotransmission in the central nervous system.
Dysregulation of KCC2 has been implicated in neurological disorders such as epilepsy, neuropathic pain, and neurodevelopmental disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Potassium Chloride Cotransporters 2 (KCC2) ELISA Kit provides researchers with a powerful tool for investigating the role of KCC2 in neurological disorders and advancing our understanding of chloride homeostasis in the brain.
Product Name:
Human Potassium Chloride Cotransporters 2 (KCC2) ELISA Kit
Product Code:
HUDL01597
Size:
96 Assays
Target:
KCC2
Synonyms:
SLC12A1, NKCC2, Solute Carrier Family 12 Member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
