Human PON1 Recombinant Protein (RPPB2144)
- SKU:
- RPPB2144
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P27169
- Research Area:
- Enzymes
Description
| Product Name: | Human PON1 Recombinant Protein |
| Product Code: | RPPB2144 |
| Size: | 10µg |
| Species: | Human |
| Target: | PON1 |
| Synonyms: | Serum paraoxonase, arylesterase 1, EC 3.1.1.2, EC 3.1.8.1, PON 1, Serum aryldialkylphosphatase 1, A-esterase 1, Aromatic esterase 1, K-45, ESA, PON. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | PON1 is supplied in 20mM Tris-HCl pH 8.0 and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 1-2 weeks.�Store, frozen at -20°C for longer periods of time.�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. Single band on Western Blot. |
| Amino Acid Sequence: | MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLRSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCELZ |
Paraoxonase 1 also called Esterase-A is involved in the detoxification of organophosphate insecticides such as parathion. Paraoxonase 1 may also confer protection against coronary artery disease by destroying proinflammatory oxidized lipids present in oxidized low-density lipoproteins (LDLs).
Paraoxonase-1 Isoform Human Recombinant is expressed in E. coli, fused to a 4.5kDa amino terminal hexahistidine tag, having a total molecular weight of 42.9kDa.The PON1 purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family. |
| UniProt Protein Details: | Protein type:Lipid-binding; EC 3.1.8.1; EC 3.1.1.2; Secreted, signal peptide; Hydrolase; Secreted; Phosphatase (non-protein); Motility/polarity/chemotaxis; EC 3.1.1.81 Chromosomal Location of Human Ortholog: 7q21.3 Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region Molecular Function:protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity Biological Process: response to nutrient levels; response to external stimulus; dephosphorylation; organophosphate catabolic process; response to toxin; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5 |
| NCBI Summary: | The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P27169 |
| NCBI GenInfo Identifier: | 308153572 |
| NCBI Gene ID: | 5444 |
| NCBI Accession: | P27169.3 |
| UniProt Secondary Accession: | P27169,Q16052, Q6B0J6, Q9UCB1, B2RA40, |
| UniProt Related Accession: | P27169 |
| Molecular Weight: | 39,731 Da |
| NCBI Full Name: | Serum paraoxonase/arylesterase 1 |
| NCBI Synonym Full Names: | paraoxonase 1 |
| NCBI Official Symbol: | PON1�� |
| NCBI Official Synonym Symbols: | ESA; PON; MVCD5�� |
| NCBI Protein Information: | serum paraoxonase/arylesterase 1; K-45; PON 1; esterase A; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1 |
| UniProt Protein Name: | Serum paraoxonase/arylesterase 1 |
| UniProt Synonym Protein Names: | Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1 |
| Protein Family: | Serum paraoxonase/arylesterase |
| UniProt Gene Name: | PON1�� |
| UniProt Entry Name: | PON1_HUMAN |
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