Human Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) ELISA Kit
The Human PABPC1L ELISA Kit is specifically designed for the precise measurement of the Poly(A)-binding protein cytoplasmic 1-like protein (PABPC1L) levels in human samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PABPC1L is a critical protein involved in mRNA stability and translation, playing a crucial role in gene expression regulation. It has been implicated in various cellular processes, including cell proliferation, differentiation, and apoptosis.
Dysregulation of PABPC1L expression has been linked to several diseases such as cancer, neurological disorders, and metabolic conditions, making it an important biomarker for investigating these conditions and potentially developing therapeutic strategies.Overall, the Human PABPC1L ELISA Kit provides researchers with a reliable tool to study the role of PABPC1L in various biological processes and diseases, paving the way for advancements in diagnostics and treatment options.
Product Name:
Human Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) ELISA Kit
Product Code:
HUDL02166
Size:
96 Assays
Target:
PABPC1L
Synonyms:
C20orf119, PABPC1L1, ePAB, Polyadenylate-Binding Protein 1-Like
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
