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Human POLR3K Recombinant Protein (RPPB2143)

SKU:
RPPB2143
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y2Y1
Research Area:
Enzymes
€309
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Description

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Product Name:Human POLR3K Recombinant Protein
Product Code:RPPB2143
Size:10µg
Species:Human
Target:POLR3K
Synonyms:DNA-directed RNA polymerase III subunit RPC10, RNA polymerase III subunit C10, DNA-directed RNA polymerase III subunit K, RNA polymerase III 12.5 kDa subunit, RPC12.5, RNA polymerase III subunit C11, HsC11p, RPC11, hRPC11, POLR3K, My010, Polymerase III Polypeptide K, C11, C11-RNP3, hRPC11, RPC10.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:POLR3K protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 50% glycerol, 2mM DTT and 2mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D

Polymerase III Polypeptide K, known as PORL3K, is a small vital subunit of RNA polymerase III. The carboxy-terminal domain of PORL3K owns a sequence which is very similar to the carboxy-terminal domain of an RNA polymerase II elongation factor. PORL3K takes part in sensing and limiting infection by intracellular bacteria and DNA viruses. PORL3K is also plays a role as nuclear and cytosolic DNA sensor which takes part in the innate immune response.

POLR3K Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 131 amino acids (1-108 a.a) and having a molecular mass of 14.7kDa.POLR3K is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:POLR3K: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
UniProt Protein Details:

Protein type:Transcription initiation complex; DNA-binding; Transferase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function:DNA binding; zinc ion binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

NCBI Summary:This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010]
UniProt Code:Q9Y2Y1
NCBI GenInfo Identifier:116242768
NCBI Gene ID:51728
NCBI Accession:Q9Y2Y1.2
UniProt Secondary Accession:Q9Y2Y1,Q1W6H4, Q96S35,
UniProt Related Accession:Q9Y2Y1
Molecular Weight:108
NCBI Full Name:DNA-directed RNA polymerase III subunit RPC10
NCBI Synonym Full Names:polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
NCBI Official Symbol:POLR3K��
NCBI Official Synonym Symbols:C11; My010; RPC10; RPC11; RPC12.5; C11-RNP3��
NCBI Protein Information:DNA-directed RNA polymerase III subunit RPC10; RNA polymerase III subunit C10; RNA polymerase III subunit C11; RNA polymerase III subunit CII; RNA polymerase III 12.5 kDa subunit; DNA-directed RNA polymerase III subunit K; DNA-directed RNA polymerases III 12.5 kDa polypeptide
UniProt Protein Name:DNA-directed RNA polymerase III subunit RPC10
UniProt Synonym Protein Names:DNA-directed RNA polymerase III subunit K; RNA polymerase III 12.5 kDa subunit; RPC12.5; RNA polymerase III subunit C11; HsC11p; RPC11; hRPC11
Protein Family:DNA-directed RNA polymerase
UniProt Gene Name:POLR3K��
UniProt Entry Name:RPC10_HUMAN

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