Human POLR2I Recombinant Protein (RPPB2140)
- SKU:
- RPPB2140
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P36954
- Research Area:
- Enzymes
Description
| Product Name: | Human POLR2I Recombinant Protein |
| Product Code: | RPPB2140 |
| Size: | 10µg |
| Species: | Human |
| Target: | POLR2I |
| Synonyms: | hRPB14.5, RPB9, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 14.5 kDa subunit, RPB14.5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | POLR2I protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE |
Polymerase II Polypeptide I (POLR2I) is a member of the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. POLR2I is a subunit of RNA polymerase II, which is a polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the 4 ribonucleoside triphosphates as substrates. In addition POLR2I synthesizes mRNA precursors and numerous functional non-coding RNAs.
POLR2I Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids (1-125 a.a.) and having a molecular mass of 17.0kDa.POLR2I is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLR2I: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Transferase; Nucleotide Metabolism - purine; RNA processing; EC 2.7.7.6 Chromosomal Location of Human Ortholog: 19q12 Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus Molecular Function:DNA binding; zinc ion binding; DNA-directed RNA polymerase activity Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; nucleotide-excision repair; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair |
| NCBI Summary: | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36954 |
| NCBI GenInfo Identifier: | 548702 |
| NCBI Gene ID: | 5438 |
| NCBI Accession: | P36954.1 |
| UniProt Related Accession: | P36954 |
| Molecular Weight: | |
| NCBI Full Name: | DNA-directed RNA polymerase II subunit RPB9 |
| NCBI Synonym Full Names: | RNA polymerase II subunit I |
| NCBI Official Symbol: | POLR2I�� |
| NCBI Official Synonym Symbols: | RPB9; hRPB14.5�� |
| NCBI Protein Information: | DNA-directed RNA polymerase II subunit RPB9 |
| UniProt Protein Name: | DNA-directed RNA polymerase II subunit RPB9 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit; RPB14.5 |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR2I�� |
| UniProt Entry Name: | RPB9_HUMAN |
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