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Human POLR2H Recombinant Protein (RPPB4273)

SKU:
RPPB4273
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P52434
€309
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Description

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Product Name:Human POLR2H Recombinant Protein
Product Code:RPPB4273
Size:20µg
Species:Human
Target:POLR2H
Synonyms:DNA-directed RNA polymerases I, II, and III subunit RPABC3, RNA polymerases I, II, and III subunit ABC3, DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, RPB17, RPB8 homolog, hRPB8, POLR2H, polymerase (RNA) II (DNA directed) polypeptide H, RPB8, RPABC3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:POLR2H protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF

POLR2H is an essential and highly conserved subunit of RNA polymerase II which is shared by the other 2 eukaryotic DNA-directed RNA polymerases, I and III. The three eukaryotic RNA polymerases are complex multisubunit enzymes which play a vital role in the transcription of nuclear genes. POLR2H encodes an essential and highly conserved subunit of RNA polymerase II which is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.

POLR2H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (1-150 a.a) and having a molecular mass of 19.5kDa.POLR2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:POLR2H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Belongs to the eukaryotic RPB8 RNA polymerase subunit family.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; Nucleolus; EC 2.7.7.6; Transferase; Nucleotide Metabolism - pyrimidine; RNA processing; Transcription initiation complex; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: cytosol; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase II, core complex; DNA-directed RNA polymerase III complex; nucleoplasm; nucleus

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of viral transcription; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair

NCBI Summary:The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
UniProt Code:P52434
NCBI GenInfo Identifier:20178325
NCBI Gene ID:5437
NCBI Accession:P52434.4
UniProt Secondary Accession:P52434,P53802, Q969R0, C9J413, C9JBJ6, C9JCU7, C9JUA8
UniProt Related Accession:P52434
Molecular Weight:9,664 Da
NCBI Full Name:DNA-directed RNA polymerases I, II, and III subunit RPABC3
NCBI Synonym Full Names:RNA polymerase II subunit H
NCBI Official Symbol:POLR2H��
NCBI Official Synonym Symbols:RPB8; RPB17; RPABC3��
NCBI Protein Information:DNA-directed RNA polymerases I, II, and III subunit RPABC3
UniProt Protein Name:DNA-directed RNA polymerases I, II, and III subunit RPABC3
UniProt Synonym Protein Names:DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; RPB17; RPB8 homolog; hRPB8
UniProt Gene Name:POLR2H��
UniProt Entry Name:RPAB3_HUMAN

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