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Human POLR2D Recombinant Protein (RPPB2136)

SKU:
RPPB2136
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O15514
Research Area:
Enzymes
€309
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Description

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Product Name:Human POLR2D Recombinant Protein
Product Code:RPPB2136
Size:20µg
Species:Human
Target:POLR2D
Synonyms:HSRBP4, HSRPB4, RBP4, RPB16, DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, POLR2D.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The POLR2D solution (1mg/ml) contains 20mM Tris-HClbuffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAAGGSD PRAGDVEEDA SQLIFPKEFE TAETLLNSEV HMLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS LEGRFEDEEL QQILDDIQTK RSFQY

Polymerase II Polypeptide D (POLR2D) is a member of the eukaryotic RPB4RNA polymerase subunit family. POLR2D is the fourth largest subunit of RNApolymerase II. Pol II is the main element of the basal RNA polymerase IItranscription machinery. polymerase is responsible forsynthesizing messenger RNA in eukaryotes. In yeast, POLR2D subunit is connectedwith the polymerase under suboptimalgrowth conditions and might have a stress protective role. POLR2D catalyzes thetranscription of DNA into RNA by the 4 ribonucleoside triphosphates assubstrates.

POLR2D Human Recombinant produced in E.coli is a single,non-glycosylated polypeptide chain containing 165 amino acids (1-142) andhaving a molecular mass of 18.7kDa.POLR2D is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:POLR2D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Belongs to the eukaryotic RPB4 RNA polymerase subunit family.
UniProt Protein Details:

Protein type:RNA processing; Transferase; EC 2.7.7.6; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function:single-stranded RNA binding; translation initiation factor binding; DNA-directed RNA polymerase activity; nucleotide binding; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; DNA repair; nuclear mRNA splicing, via spliceosome; positive regulation of translational initiation; mRNA export from nucleus during heat stress; mRNA capping; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; mRNA catabolic process, deadenylation-dependent decay

NCBI Summary:This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O15514
NCBI GenInfo Identifier:3024558
NCBI Gene ID:5433
NCBI Accession:O15514.1
UniProt Related Accession:O15514
Molecular Weight:
NCBI Full Name:DNA-directed RNA polymerase II subunit RPB4
NCBI Synonym Full Names:RNA polymerase II subunit D
NCBI Official Symbol:POLR2D��
NCBI Official Synonym Symbols:RBP4; RPB16; HSRBP4; HSRPB4��
NCBI Protein Information:DNA-directed RNA polymerase II subunit RPB4
UniProt Protein Name:DNA-directed RNA polymerase II subunit RPB4
UniProt Synonym Protein Names:DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit; RPB16
Protein Family:Transcriptional adapter
UniProt Gene Name:POLR2D��
UniProt Entry Name:RPB4_HUMAN

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