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Human POLR1C Recombinant Protein (RPPB4271)

SKU:
RPPB4271
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O15160
€309
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Description

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Product Name:Human POLR1C Recombinant Protein
Product Code:RPPB4271
Size:10µg
Species:Human
Target:POLR1C
Synonyms:DNA-directed RNA polymerases I and III subunit RPAC1, POLR1C, POLR1E, RPC40, Polymerase (RNA) I (DNA directed) Polypeptide C, RPA40, RPA39, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RNA polymerases I and III subunit AC1, AC40, DNA-directed RNA polymerase I subunit C, RP3-337H4.4, RPA5, RPAC1, TCS3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:POLR1C protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAASQAV EEMRSRVVLG EFGVRNVHTT DFPGNYSGYD DAWDQDRFEK NFRVDVVHMD ENSLEFDMVG IDAAIANAFR RILLAEVPTM AVEKVLVYNN TSIVQDEILA HRLGLIPIHA DPRLFEYRNQ GDEEGTEIDT LQFRLQVRCT RNPHAAKDSS DPNELYVNHK VYTRHMTWIP LGNQADLFPE GTIRPVHDDI LIAQLRPGQE IDLLMHCVKG IGKDHAKFSP VATASYRLLP DITLLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDVLVSEAIK VLMGKCRRFL DELDAVQMD

Polymerase (RNA) I (DNA directed) Polypeptide C (POLR1C) is a subunit of both RNA polymerase I and RNA polymerase III complexes whose defects are related with Collins syndrome. POLR1C is a protein-coding gene and is part of the Pol core element

POLR1C Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 369 amino acids (1-346 a.a.) and having a molecular mass of 41.6kDa.POLR1C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transferase; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transcription initiation complex

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol; DNA-directed RNA polymerase I complex

Molecular Function:protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; negative regulation of gene expression, epigenetic; positive regulation of interferon type I production; transcription from RNA polymerase I promoter; innate immune response; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3

NCBI Summary:The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). [provided by RefSeq, Mar 2011]
UniProt Code:O15160
NCBI GenInfo Identifier:3914807
NCBI Gene ID:9533
NCBI Accession:O15160.1
UniProt Secondary Accession:O15160,O75395, Q5JTE3,
UniProt Related Accession:O15160
Molecular Weight:346
NCBI Full Name:DNA-directed RNA polymerases I and III subunit RPAC1
NCBI Synonym Full Names:polymerase (RNA) I polypeptide C, 30kDa
NCBI Official Symbol:POLR1C��
NCBI Official Synonym Symbols:RPA5; TCS3; RPA39; RPA40; RPAC1��
NCBI Protein Information:DNA-directed RNA polymerases I and III subunit RPAC1; AC40; RPC40; RNA polymerases I and III subunit AC1; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide
UniProt Protein Name:DNA-directed RNA polymerases I and III subunit RPAC1
UniProt Synonym Protein Names:AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40
Protein Family:DNA-directed RNA polymerases
UniProt Gene Name:POLR1C��
UniProt Entry Name:RPAC1_HUMAN

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