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Human Poliovirus Receptor Related Protein 4 (PVRL4) ELISA Kit

SKU:
HUDL02470
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q96NY8
Sensitivity:
52pg/mL
Range:
125-8000pg/mL
ELISA Type:
Sandwich
Synonyms:
PRR4,LNIR,Nectin-4,Ig superfamily receptor LNIR,Processed poliovirus receptor-related protein 4
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Poliovirus Receptor Related Protein 4 (PVRL4) ELISA Kit

The Human Poliovirus Receptor Related Protein 4 (PVRL4) ELISA Kit is a reliable tool for the sensitive detection of PVRL4 levels in human samples including serum, plasma, and cell culture supernatants. With high specificity and accuracy, this kit provides consistent and reproducible results, making it suitable for a variety of research applications.PVRL4, also known as nectin-4, is a cell adhesion molecule that plays a crucial role in various cellular processes including cell-cell adhesion and signaling.

Its expression has been implicated in cancer progression, particularly in breast and ovarian cancers, making it a valuable biomarker for studying tumorigenesis and developing targeted therapies.With its user-friendly design and robust performance, the Human PVRL4 ELISA Kit is the ideal choice for researchers looking to investigate the role of PVRL4 in cancer and other diseases, facilitating advancements in the field of molecular biology and personalized medicine.

Product Name:Human Poliovirus Receptor Related Protein 4 (PVRL4) ELISA Kit
Product Code:HUDL02470
Size:96 Assays
Target:PVRL4
Synonyms:PRR4, LNIR, Nectin-4, Ig superfamily receptor LNIR, Processed poliovirus receptor-related protein 4
Detection Method:Sandwich
Range:125-8,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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