Human POLE3 Recombinant Protein (RPPB4270)
- SKU:
- RPPB4270
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NRF9
Description
| Product Name: | Human POLE3 Recombinant Protein |
| Product Code: | RPPB4270 |
| Size: | 20µg |
| Species: | Human |
| Target: | POLE3 |
| Synonyms: | CHARAC17, CHRAC17, p17, YBL1,DNA polymerase epsilon subunit 3, Arsenic-transactivated protein,AsTP, Chromatin accessibility complex 17 kDa protein, HuCHRAC17, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, POLE3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The POLE3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN |
Polymerase (DNA Directed), Epsilon 3, also know as POLE3, is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers merge into larger enzymatic complexes for DNA transcription, replication, and packaging.
POLE3 Human Recombinant produced in E. coli is a single polypeptide chain containing 170 amino acids (1-147) and having a molecular mass of 19kDa. POLE3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; Nucleotide Metabolism - purine; Transferase Chromosomal Location of Human Ortholog: 9q33 Cellular Component: epsilon DNA polymerase complex; nucleus Molecular Function:protein binding Biological Process: DNA replication |
| NCBI Summary: | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
| UniProt Code: | Q9NRF9 |
| NCBI GenInfo Identifier: | 22653710 |
| NCBI Gene ID: | 54107 |
| NCBI Accession: | Q9NRF9.1 |
| UniProt Secondary Accession: | Q9NRF9,Q5W0U1, Q8N758, Q8NCE5, Q9NR32, |
| UniProt Related Accession: | Q9NRF9 |
| Molecular Weight: | 16,860 Da |
| NCBI Full Name: | DNA polymerase epsilon subunit 3 |
| NCBI Synonym Full Names: | DNA polymerase epsilon 3, accessory subunit |
| NCBI Official Symbol: | POLE3�� |
| NCBI Official Synonym Symbols: | p17; YBL1; CHRAC17; CHARAC17�� |
| NCBI Protein Information: | DNA polymerase epsilon subunit 3 |
| UniProt Protein Name: | DNA polymerase epsilon subunit 3 |
| UniProt Synonym Protein Names: | Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17 |
| Protein Family: | DNA polymerase |
| UniProt Gene Name: | POLE3�� |
| UniProt Entry Name: | DPOE3_HUMAN |
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