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Human PMM1 Recombinant Protein (RPPB2127)

SKU:
RPPB2127
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q92871
Research Area:
Enzymes
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Description

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Product Name:Human PMM1 Recombinant Protein
Product Code:RPPB2127
Size:10µg
Species:Human
Target:PMM1
Synonyms:Phosphomannomutase 1, PMM 1, PMMH-22, PMM1, PMMH22, Sec53.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PMM1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT, 100mM NaCl and 0.1mM PMSF.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA

Phosphomannomutase 1 (PMM1) is an enzyme involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM1 catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for the synthesis of dolichol-phosphate-mannose, which is crucial for N-linked glycosylation and accordingly the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. Additionally, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.

PMM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 282 amino acids (1-262 a.a.) and having a molecular mass of 31.9kDa. The PMM1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:PMM1: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain. Belongs to the eukaryotic PMM family.Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; EC 5.4.2.8; IsomeraseChromosomal Location of Human Ortholog: 22q13.2Cellular Component: cytosolMolecular Function: phosphomannomutase activity; protein bindingBiological Process: GDP-mannose biosynthetic process; mannose metabolic process; protein amino acid N-linked glycosylation; protein targeting to ER
UniProt Protein Details:
NCBI Summary:Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q92871
NCBI GenInfo Identifier:194097356
NCBI Gene ID:5372
NCBI Accession:NP_002667.2
UniProt Secondary Accession:Q92871,Q92586, A8K003
UniProt Related Accession:Q92871
Molecular Weight:29,747 Da
NCBI Full Name:phosphomannomutase 1
NCBI Synonym Full Names:phosphomannomutase 1
NCBI Official Symbol:PMM1��
NCBI Official Synonym Symbols:Sec53��
NCBI Protein Information:phosphomannomutase 1
UniProt Protein Name:Phosphomannomutase 1
UniProt Synonym Protein Names:PMMH-22
Protein Family:
UniProt Gene Name:PMM1��
UniProt Entry Name:PMM1_HUMAN

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