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Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1) ELISA Kit (HUEB1654)

SKU:
HUEB1654
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q53GL0
Range:
0.625-40 ng/ml
ELISA Type:
Sandwich
Synonyms:
PLEKHO1, Pleckstrin homology domain-containing family O member 1, PH domain-containing family O member 1
Reactivity:
Human
$779
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Description

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Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1) ELISA Kit

The Human Pleckstrin Homology Domain Containing Family O Member 1 (PLEKHO1) ELISA Kit is a valuable tool for accurately measuring PLEKHO1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results that are essential for a variety of research applications.PLEKHO1 is a protein that contains a pleckstrin homology (PH) domain and is involved in various cellular processes, including cell adhesion and migration.

Abnormal levels of PLEKHO1 have been associated with certain diseases, such as cancer and inflammatory conditions, making it an important biomarker for studying these ailments and potentially developing new therapeutic strategies.Overall, the Human PLEKHO1 ELISA Kit is a reliable tool for researchers looking to study the role of PLEKHO1 in health and disease, providing accurate and reproducible results that can aid in furthering our understanding of this important protein.

Product Name:Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1) ELISA Kit
SKU:HUEB1654
Size:96T
Target:Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1)
Synonyms:C-Jun-binding protein, Casein kinase 2-interacting protein 1, Osteoclast maturation-associated gene 120 protein, JBP, CK2-interacting protein 1, HQ0024c, PH domain-containing family O member 1, CKIP1, OC120
Assay Type:Competitive
Detection Method:ELISA
Reactivity:Human
Detection Range:0.625-40ng/ml
Sensitivity:0.21ng/ml
Intra CV:4.9%
Inter CV:7.4%
Linearity:
Sample1:21:41:81:16
Serum(N=5)107-119%104-113%104-114%86-96%
EDTA Plasma(N=5)114-123%82-90%94-104%99-111%
Heparin Plasma(N=5)106-116%95-104%106-116%97-107%
Recovery:
Sample TypeAverage(%)Recovery Range(%)
Serum9892-104
Plasma10094-106
Function:Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
Uniprot:Q53GL0
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant human Pleckstrin homology domain-containing family O member 1
Sub Unit:Heterodimer or homodimer. Interacts with CK2 and actin capping subunits (capping protein CP-alpha and CP-beta). CKIP1 and CK2 together inhibit the activity of actin capping protein at the barbed ends of actin filaments. Interacts with ATM, IFP35, JUN, JUND, NMI and PI3K. Interacts with AKT1, AKT2 and AKT3 (each isozyme of PKB), PtdIns(3,5)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P2.
Subcellular Location:Cell membrane Peripheral membrane protein Nucleus Cytoplasm Predominantly localized to the plasma membrane through the binding to phosphatidylinositol 3-phosphate (PubMed:14729969). In C2C12 cells, with the absence of growth factor, it is found in the nucleus (PubMed:14729969). It rapidly translocates to the plasma membrane after insulin stimulation (PubMed:14729969). In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3 (PubMed:15706351). However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells (Probable).
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 1q21.2

Molecular Function:protein binding

UniProt Code:Q53GL0
NCBI GenInfo Identifier:160419242
NCBI Gene ID:51177
NCBI Accession:Q53GL0.2
UniProt Secondary Accession:Q53GL0,Q336K5, Q8IZ51, Q9NRV3, Q9UL48,
UniProt Related Accession:Q53GL0
Molecular Weight:42,330 Da
NCBI Full Name:Pleckstrin homology domain-containing family O member 1
NCBI Synonym Full Names:pleckstrin homology domain containing O1
NCBI Official Symbol:PLEKHO1
NCBI Official Synonym Symbols:JBP; CKIP1; OC120; CKIP-1
NCBI Protein Information:pleckstrin homology domain-containing family O member 1
UniProt Protein Name:Pleckstrin homology domain-containing family O member 1
UniProt Synonym Protein Names:C-Jun-binding protein; JBP; Casein kinase 2-interacting protein 1; CK2-interacting protein 1; CKIP-1; Osteoclast maturation-associated gene 120 protein
Protein Family:Pleckstrin homology domain-containing family
UniProt Gene Name:PLEKHO1
UniProt Entry Name:PKHO1_HUMAN
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.
ELISA
Human PLEKHO1 / Pleckstrin homology domain-containing family O member 1 ELISA Kit

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