Human Platelet Membrane Glycoprotein IV (GP4) ELISA Kit
SKU:
HUDL01226
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P16671
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD36,FAT,GP3B,GPIV,PASIV,SCARB3,GP88,GpIIIb,Glycoprotein IIIb,Collagen Type I Receptor,Thrombospondin Receptor,Fatty acid translocase,Platelet collagen receptor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Human Platelet Membrane Glycoprotein IV (GP4) ELISA Kit
The Human Platelet Membrane Glycoprotein IV (GP4) ELISA Kit is a cutting-edge tool designed for the accurate detection of GP4 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an invaluable asset for various research applications.GP4, also known as CD36, is a vital glycoprotein present on platelet membranes that plays a crucial role in thrombosis and hemostasis. Additionally, GP4 is involved in various physiological processes, including lipid metabolism and angiogenesis, making it a key player in cardiovascular health and disease.
By accurately measuring GP4 levels, researchers can gain valuable insights into platelet function, cardiovascular health, and potential therapeutic targets for various disorders. The Human Platelet Membrane Glycoprotein IV (GP4) ELISA Kit is a must-have tool for studies related to platelet biology, cardiovascular diseases, and lipid metabolism.
Product Name:
Human Platelet Membrane Glycoprotein IV (GP4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
