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Human Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

SKU:
HUDL02237
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01127
Sensitivity:
11.3pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDGF-B,PDGF2,SIS,SSV,C-sis,Simian Sarcoma Viral(v-sis)Oncogene Homolog,Platelet Derived Growth Factor Beta Polypeptide,Proto-oncogene c-Sis,Becaplermin
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

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Human Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

The Human Platelet-Derived Growth Factor Subunit B (PDGFB) ELISA Kit is a valuable tool for accurately measuring PDGFB levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.PDGFB is a key growth factor known for its role in cell growth and division, particularly in the formation of blood vessels. Its dysregulation has been implicated in various diseases, including cancer and cardiovascular disorders.

By accurately measuring PDGFB levels, researchers can gain insights into the mechanisms underlying these conditions and potentially develop targeted therapies.Overall, the Human PDGFB ELISA Kit offers researchers a reliable and efficient method for quantifying PDGFB levels, advancing our understanding of its physiological and pathological roles in health and disease.

Product Name:Human Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit
Product Code:HUDL02237
Size:96 Assays
Target:PDGFB
Synonyms:PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral(v-sis)Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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