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Human Platelet Derived Growth Factor Receptor Beta (PDGFRb) ELISA Kit

SKU:
HUDL02241
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09619
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD140b,JTK12,PDGF-R-Beta,PDGFR,PDGFR1,CD140 antigen-like family member B,Platelet-derived growth factor receptor 1,Beta-type platelet-derived growth factor receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€599
Frequently bought together:

Description

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Human Platelet Derived Growth Factor Receptor Beta (PDGFRb) ELISA Kit

The Human Platelet-Derived Growth Factor Receptor Beta (PDGFRB) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of PDGFRB levels in human serum, plasma, and cell culture supernatants. This kit is essential for research applications, providing reliable and consistent results.PDGFRB is a key receptor involved in cell growth, differentiation, and migration, making it a crucial player in various physiological and pathological processes.

Dysregulation of PDGFRB has been implicated in diseases such as cancer and fibrosis, highlighting its importance as a potential therapeutic target and biomarker.With its high-quality components and proven performance, the Human PDGFRB ELISA Kit is a valuable tool for researchers studying signaling pathways, drug development, and disease mechanisms related to PDGFRB. Trust in this kit to deliver accurate and reproducible data for your research needs.

Product Name:Human Platelet Derived Growth Factor Receptor Beta (PDGFRb) ELISA Kit
Product Code:HUDL02241
Size:96 Assays
Target:PDGFRb
Synonyms:CD140b, JTK12, PDGF-R-Beta, PDGFR, PDGFR1, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, Beta-type platelet-derived growth factor receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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