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Human Plasminogen Activator, Urokinase (uPA) ELISA Kit

SKU:
HUDL03054
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00749
Sensitivity:
7.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
PLAU,ATF,URK,UK,UP-A,Abbokinase,Urokinase-Type Plasminogen Activator
Reactivity:
Human
Sample Type:
Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

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Human Plasminogen Activator, Urokinase (uPA) ELISA Kit

The Human Plasminogen Activator Urokinase (UPA) ELISA Kit is specifically designed for the accurate quantification of UPA levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.UPA is a key enzyme that plays a critical role in fibrinolysis, the process of breaking down blood clots. Dysregulation of UPA has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.

The Human UPA ELISA Kit provides researchers with a reliable tool for studying these disease mechanisms and exploring potential therapeutic interventions.With its advanced technology and user-friendly protocols, the Human Plasminogen Activator Urokinase ELISA Kit is an indispensable resource for scientists seeking to investigate the role of UPA in health and disease.

Product Name:Human Plasminogen Activator, Urokinase (uPA) ELISA Kit
Product Code:HUDL03054
Size:96 Assays
Target:uPA
Synonyms:PLAU, ATF, URK, UK, UP-A, Abbokinase, Urokinase-Type Plasminogen Activator
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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