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Human Placental Protein 13 (PP13) ELISA Kit

SKU:
HUDL02370
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UHV8
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
LGALS13,PLAC8,PP13,GAL13,Galectin 13,Galactoside-Binding Soluble Lectin 13,Placental tissue protein 13
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Placental Protein 13 (PP13) ELISA Kit

The Human Placental Protein 13 (PP13) ELISA Kit is specifically designed for the accurate detection of PP13 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for researchers in various fields.PP13 is a protein found in the placenta and is known to be involved in various pregnancy-related processes, including fetal growth and development.

Abnormal levels of PP13 have been linked to pregnancy complications such as preeclampsia, making it an important biomarker for monitoring and studying these conditions.Overall, the Human Placental Protein 13 (PP13) ELISA Kit offers researchers a precise and efficient method for measuring PP13 levels, allowing for a better understanding of its role in pregnancy and potential implications for maternal and fetal health.

Product Name:Human Placental Protein 13 (PP13) ELISA Kit
Product Code:HUDL02370
Size:96 Assays
Target:PP13
Synonyms:LGALS13, PLAC8, PP13, GAL13, Galectin 13, Galactoside-Binding Soluble Lectin 13, Placental tissue protein 13
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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