Human PLA2R1 (Phospholipase A2 Receptor 1) CLIA Kit
The Human PLA2R1 (Phospholipase A2 Receptor 1) CLIA Kit is an advanced assay designed for the accurate detection of PLA2R1 levels in human serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.PLA2R1 is a key receptor involved in phospholipase A2 signaling pathways, playing a critical role in inflammatory responses and lipid metabolism regulation. Dysregulation of PLA2R1 has been associated with autoimmune diseases, kidney disorders, and other pathological conditions, making it a valuable biomarker for studying these diseases and developing targeted interventions.
With its innovative technology and user-friendly design, the Human PLA2R1 CLIA Kit is a valuable tool for researchers and clinicians seeking to explore the role of PLA2R1 in health and disease. Order now to enhance your research capabilities and advance scientific understanding in this important field.
Product Name:
Human PLA2R1 (Phospholipase A2 Receptor 1) CLIA Kit
SKU:
HUES00625
Target:
Human PLA2R1 (Phospholipase A2 Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLA2R1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLA2R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLA2R1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLA2R1. You can calculate the concentration of Human PLA2R1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
88-102
85-98
Average (%)
95
95
91
1:4
Range (%)
102-115
97-112
95-107
Average (%)
109
105
101
1:8
Range (%)
94-107
84-98
96-109
Average (%)
101
91
103
1:16
Range (%)
92-105
100-116
85-98
Average (%)
99
107
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
102-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.17
304.56
900.12
99.51
278.42
875.37
Standard deviation
9.72
30.61
69.49
12.72
21.69
74.41
C V (%)
10.55
10.05
7.72
12.78
7.79
8.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PLA2R1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLA2R1 in samples. No significant cross-reactivity or interference between Human PLA2R1 and analogues was observed.
