Human PLA2G10 Recombinant Protein (RPPB2118)
- SKU:
- RPPB2118
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O15496
- Research Area:
- Enzymes
Description
| Product Name: | Human PLA2G10 Recombinant Protein |
| Product Code: | RPPB2118 |
| Size: | 10µg |
| Species: | Human |
| Target: | PLA2G10 |
| Synonyms: | Group 10 secretory phospholipase A2, EC 3.1.1.4, Group X secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase GX, GX sPLA2, sPLA2-X, SPLA2, GXPLA2, MGC119918, MGC119919, MGC133367, PLA2G10. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered lyophilized (freeze-dried) powder. |
| Formulation: | PLA2G10 filtered (0.4 �m) and lyophilized from 0.5mg/ml solution in 20mM Tris and 50mM NaCl, pH 7.5. |
| Solubility: | It is recommended to add 200�l of deionized waterto prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95% as determined by SDS PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASHMGILE LAGTVGCVGP RTPIAYMKYG CFCGLGGHGQ PRDAIDWCCH GHDCCYTRAE EAGCSPKTER YSWQCVNQSV LCGPAENKCQ ELLCKCDQEI ANCLAQTEYN LKYLFYPQFL CEPDSPKCD |
Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.
Secreted Phospholipase A2-X Human Recombinant is manufactured with N-terminal fusion HisTag. PLA2G10 His-Tagged Fusion Protein, is 15.5 kDa containing 123 amino acid residues of the human secreted phospholipase A2-X and 16 additional amino acid residues - HisTag (underlined).
| UniProt Protein Function: | PLA2G10: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. Belongs to the phospholipase A2 family. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis; Phospholipase; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 16p13.12 Cellular Component: extracellular region Molecular Function:phospholipase A2 activity; phospholipase activity Biological Process: axon guidance; cholesterol homeostasis; lysophospholipid transport; negative regulation of transcription factor activity; phosphatidic acid biosynthetic process; positive regulation of cellular protein metabolic process; positive regulation of prostaglandin secretion; regulation of macrophage activation |
| NCBI Summary: | This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism. [provided by RefSeq, Nov 2015] |
| UniProt Code: | O15496 |
| NCBI GenInfo Identifier: | 239938869 |
| NCBI Gene ID: | 8399 |
| NCBI Accession: | O15496.3 |
| UniProt Secondary Accession: | O15496,Q14DU3, Q6NT23, |
| UniProt Related Accession: | O15496 |
| Molecular Weight: | 18kDa |
| NCBI Full Name: | Group 10 secretory phospholipase A2 |
| NCBI Synonym Full Names: | phospholipase A2 group X |
| NCBI Official Symbol: | PLA2G10�� |
| NCBI Official Synonym Symbols: | SPLA2; GXPLA2; GXSPLA2; sPLA2-X�� |
| NCBI Protein Information: | group 10 secretory phospholipase A2 |
| UniProt Protein Name: | Group 10 secretory phospholipase A2 |
| UniProt Synonym Protein Names: | Group X secretory phospholipase A2; GX sPLA2; sPLA2-X; Phosphatidylcholine 2-acylhydrolase 10 |
| Protein Family: | Group 10 secretory phospholipase |
| UniProt Gene Name: | PLA2G10�� |
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