The Human PKM2 (Pyruvate Kinase Muscle) CLIA Kit is a robust and reliable assay designed for the accurate detection of PKM2 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and consistent results, making it suitable for various research applications.PKM2 is an important enzyme involved in cancer metabolism, regulating the glycolysis pathway and influencing tumor growth and progression.
It is also known to play a role in other diseases such as cardiovascular disorders and metabolic disorders. By accurately measuring PKM2 levels, researchers can gain valuable insights into disease pathogenesis and potential therapeutic strategies.Overall, the Human PKM2 CLIA Kit is a valuable tool for studying the role of PKM2 in cancer and other diseases, offering researchers a reliable method for quantifying PKM2 levels and advancing their research efforts.
Product Name:
Human PKM2 (Pyruvate Kinase, Muscle) CLIA Kit
SKU:
HUES00639
Target:
Human PKM2 (Pyruvate Kinase, Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PKM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PKM2. You can calculate the concentration of Human PKM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
88-102
88-102
Average (%)
106
94
95
1:4
Range (%)
92-110
89-101
91-106
Average (%)
100
95
97
1:8
Range (%)
97-112
90-102
104-116
Average (%)
104
95
109
1:16
Range (%)
89-101
97-112
86-100
Average (%)
96
105
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
88-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.31
145.07
456.58
57.29
131.22
416.35
Standard deviation
5.76
14.72
35.11
5.63
9.25
28.56
C V (%)
10.41
10.15
7.69
9.83
7.05
6.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PKM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PKM2 in samples. No significant cross-reactivity or interference between Human PKM2 and analogues was observed.
