Human Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) ELISA Kit
The Human PTTG1IP (Pituitary Tumor-Transforming 1 Interacting Protein) ELISA Kit is specifically designed for the precise measurement of PTTG1IP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research applications.PTTG1IP is a key protein involved in cell division and proliferation, playing a critical role in tumorigenesis and tumor progression. Dysregulation of PTTG1IP has been implicated in various cancers and other diseases, making it a crucial biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human PTTG1IP ELISA Kit, researchers can gain valuable insights into the role of PTTG1IP in disease pathogenesis and progression, paving the way for advancements in diagnosis, prognosis, and targeted therapy development. Trust in the reliability and performance of this kit for precise and accurate measurements of PTTG1IP levels in human samples.
Product Name:
Human Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
