Human P1NP (Procollagen I N-terminal Propeptide ) ELISA Kit
The Human PINP (Procollagen I N-Terminal Propeptide) ELISA Kit is specifically designed for the quantitative detection of PINP levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.PINP is a key marker for collagen synthesis and bone formation, serving as a vital indicator of bone turnover and health. Elevated PINP levels are associated with conditions like osteoporosis, fractures, and other bone diseases, making it a valuable biomarker for studying bone metabolism and assessing the efficacy of therapeutic interventions.
With its reliable performance and user-friendly protocols, the Human PINP ELISA Kit is an essential tool for researchers and healthcare professionals seeking to study bone health, monitor disease progression, and develop targeted treatment strategies.
Product Name:
Human P1NP (Procollagen I N-terminal Propeptide ) ELISA Kit
SKU:
HUES01452
Target:
Human P1NP (Procollagen I N-terminal Propeptide )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Pâ… NP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pâ… NP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pâ… NP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Pâ… NP. You can calculate the concentration of Human Pâ… NP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
88-99
89-104
Average (%)
100
93
96
1:4
Range (%)
91-103
86-99
83-95
Average (%)
96
92
90
1:8
Range (%)
86-100
85-98
88-103
Average (%)
92
90
94
1:16
Range (%)
90-105
85-96
85-97
Average (%)
96
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
90
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.12
128.38
437.51
54.81
139.67
400.58
Standard deviation
3.25
6.02
18.51
3.47
6.13
17.95
C V (%)
6.24
4.69
4.23
6.33
4.39
4.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Pâ… NP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pâ… NP in samples. No significant cross-reactivity or interference between Human Pâ… NP and analogues was observed.
