Human P1NP (Procollagen I N-terminal Propeptide ) CLIA Kit
The Human PINP (Procollagen I N-terminal propeptide) CLIA Kit is specifically designed for the accurate measurement of PINP levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research purposes.PINP is a key marker of collagen synthesis and is essential for the formation of connective tissue and bone. Elevated levels of PINP have been associated with conditions such as osteoporosis, bone disorders, and bone metastasis, making it a valuable biomarker for studying these diseases and developing targeted therapies.
With its advanced technology and reliable performance, the Human PINP CLIA Kit is an indispensable tool for researchers in the fields of bone biology, osteoporosis, and connective tissue disorders. Trust in this kit to deliver accurate results for your important research projects.
Product Name:
Human P1NP (Procollagen I N-terminal Propeptide ) CLIA Kit
SKU:
HUES00159
Target:
Human P1NP (Procollagen I N-terminal Propeptide )
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PINP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PINP. You can calculate the concentration of Human PINP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
94-108
94-108
Average (%)
104
101
101
1:4
Range (%)
87-100
91-106
93-108
Average (%)
92
98
100
1:8
Range (%)
102-116
90-103
87-100
Average (%)
109
95
93
1:16
Range (%)
97-109
94-108
89-104
Average (%)
103
102
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
104
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
97-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.27
60.84
167.08
23.63
63.13
176.21
Standard deviation
2.84
5.4
11.85
2.98
5.8
17.85
C V (%)
12.2
8.88
7.09
12.61
9.19
10.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PINP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PINP in samples. No significant cross-reactivity or interference between Human PINP and analogues was observed.
