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Human PIN4 Recombinant Protein (RPPB2115)

SKU:
RPPB2115
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y237
Research Area:
Enzymes
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Description

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Product Name:Human PIN4 Recombinant Protein
Product Code:RPPB2115
Size:20µg
Species:Human
Target:PIN4
Synonyms:Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, Parvulin-14, Par14, hPar14, Parvulin-17, Par17, hPar17, Peptidyl-prolyl cis-trans isomerase Pin4, PPIase Pin4, Peptidyl-prolyl cis/trans isomerase EPVH, hEPVH, Rotamase Pin4, PIN4, EPVH, MGC138486.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:PIN4 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 1mM DTT and 0.1mM PMSF.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK
Biological Activity:Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-HCl pH8.0 using chymotrypsin.

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (PIN4) is a peptidyl-prolyl cis/trans isomerase (PPIase) which interacts with NIMA and is vital for cell cycle regulation. PIN4 has 2 different isoforms: PAR14 and PAR17. Furthermore, Pin4 protein binds to double-stranded DNA under physiological salt conditions.

PIN4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (1-156 a.a.) and having a molecular mass of 18.8kDa.PIN4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Nucleolus; Isomerase; Mitochondrial; EC 5.2.1.8

Chromosomal Location of Human Ortholog: Xq13

Molecular Function:protein binding

NCBI Summary:This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]
UniProt Code:Q9Y237
NCBI GenInfo Identifier:20139299
NCBI Gene ID:5303
NCBI Accession:Q9Y237.1
UniProt Secondary Accession:Q9Y237,Q0D2H3, Q3MHV0, Q52M21, Q5HYW6, Q6IRW4, A8E0G6 B3KXM0, F5H1P5,
UniProt Related Accession:Q9Y237
Molecular Weight:14,179 Da
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
NCBI Synonym Full Names:peptidylprolyl cis/trans isomerase, NIMA-interacting 4
NCBI Official Symbol:PIN4��
NCBI Official Synonym Symbols:EPVH; PAR14; PAR17��
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
UniProt Synonym Protein Names:Parvulin-14; Par14; hPar14; Parvulin-17; Par17; hPar17; Peptidyl-prolyl cis-trans isomerase Pin4; PPIase Pin4; Peptidyl-prolyl cis/trans isomerase EPVH; hEPVH; Rotamase Pin4
Protein Family:Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:PIN4��
UniProt Entry Name:PIN4_HUMAN

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