null

Human Pim-2 Oncogene (PIM2) ELISA Kit

SKU:
HUDL02295
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9P1W9
Sensitivity:
0.227ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
Serine/threonine-protein kinase pim-2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human Pim-2 Oncogene (PIM2) ELISA Kit

The Human PIM-2 Oncogene (PIM2) ELISA Kit is a powerful tool designed for the accurate quantification of PIM-2 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reliable results for a variety of research applications.PIM-2 is a key oncogene involved in cell proliferation, survival, and differentiation, making it a valuable target for studying cancer and potential therapeutic interventions. Its dysregulation has been implicated in various types of cancers, highlighting the significance of PIM-2 as a biomarker for cancer research and drug development.

With the Human PIM-2 Oncogene (PIM2) ELISA Kit, researchers can confidently measure PIM-2 levels in biological samples, advancing our understanding of cancer biology and facilitating the development of targeted therapies. Count on this kit for accurate and reproducible results in your research endeavors.

Product Name:Human Pim-2 Oncogene (PIM2) ELISA Kit
Product Code:HUDL02295
Size:96 Assays
Target:PIM2
Synonyms:Serine/threonine-protein kinase pim-2
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products